Agarose staining

Dr. J.P. Clewley jclewley at crc.ac.uk
Tue Feb 15 17:46:00 EST 1994


In article <2jr4u9$6q8 at gabriel.keele.ac.uk>, pha23 at cc.keele.ac.uk (CWIS manager) writes:
> 
> Can anybody give an ignorant physicist some sensible ideas about the best
> methods (preferably with recipes) which we might be able to use to stain
> agarose electrophoresis gels in such a way that we can distinguish single
> stranded from double stranded DNA. We are currently using EtBr to stain all
> our gels and have tried to use Sigma "Stains All". The Stains all does seem
> to help in monitoring terminal transferase reactions but I'm not sure that
> we have the best recipe. Anybody got any useful advice?
> Trevor Forsyth, Physics Dept., Keele University, UK

You might bee able to use acridine orange; I used it years ago to distinguish
ss from ds DNA, but I don't have any current recipes.

Jon Clewley




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