PEG : to autoclave or not to autoclave, that is the ques

gc genecutl at mendel.berkeley.edu
Tue Feb 15 11:48:45 EST 1994


In article <1994Feb15.085613.7863 at abo.fi>, JANIEMI at FINABO.ABO.FI (Jarmo
Niemi) wrote:

> In <2jou5u$lpk at mule.fhcrc.org> Tim writes:
> 
> > Is there a reason why protocols for PEG precipitation of phage specify
> > that it should be filter sterilized? 
> > What's supposed to happen if you autoclave it?????
> 
> In my experience, solutions with PEG and NaCl, even when autoclaved
> separately, turn yellow after some time. Filtered PEG-NaCl solutions
> seem to do this much more slowly. What happens, I don't know, and DNA
> prepared with yellow PEG-NaCl can be sequenced, IMH (In my hands...).
> 
> Jarmo Niemi   Biochemistry, University of Turku, Finland, janiemi at finabo.abo.fi




I've heard that molecules like PEG and SDS break down when they're 
autoclaved.
I never bother to sterilize them anyway unless I'm going to use them
for E. coli work.




-- 
--gc



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