High Efficiency Transformation (libraries)
jgraham at bronze.ucs.indiana.edu
Wed Feb 16 11:11:27 EST 1994
Has anyone actually achieved transformation efficiencies near the
10(9)/ ug reported by Hanahan ? This would require a CsCl purified
spec quantitated control plasmid.
Are there any variations reported in the last several years that
are working well ?
I am intersted in making libraies from a relatively inefficient
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