High Efficiency Transformation (libraries)

the End jgraham at bronze.ucs.indiana.edu
Wed Feb 16 11:11:27 EST 1994


Has anyone actually achieved transformation efficiencies near the 
10(9)/ ug reported by Hanahan ? This would require a CsCl purified 
spec quantitated control plasmid.

Are there any variations reported in the last several years that 
are working well ? 

I am intersted in making libraies from a relatively inefficient 
ligation.

Thanks much,

Jim
J. Graham



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