Boehringer's DIG detection system.

Harald Osmundsen haraldo at karius.uio.no
Wed Feb 16 11:11:51 EST 1994


We have for a year now used Boehringer Dig system for detections of hybridizations signals in Northern blots.

We use tailed oligos as probes, and have used HPLC cleaned probes.

Has anyone tried using non-HPLC cleaned oligo- probes with this detection system?

We had one attempt recently and this was unsuccessful. Is there anyone who has had
a similar experience?


             Harald Osmundsen




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