Fuzzy Sequencing Gel Bands

Jim Owens jow at helix.nih.gov
Fri Feb 18 12:37:28 EST 1994


In article <1994Feb17.211513.26730 at emba.uvm.edu> Brian Foley,
brianf at med.uvm.edu writes:
>	Other than that, I can say that I have never had good luck loading 
>gels after too long of a pre-run.  If I need to read far from the primer
>I always pour a second gel for the long run.  I have seen my gels
>breaking down in a small region between where the upper buffer cools
>the gel and the aluminum plate that distributes heat in the middle
>of the gel.  My apparatus has a slight gap there where the gel overheats
>and gets destroyed.

For what it's worth, this has been my experience as well.

Good luck,

Jim Owens



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