Sequencing Glycogen ppt DNA?
D_henrique at icrf.icnet.uk
Fri Feb 18 06:58:26 EST 1994
In article <1994Feb17.190959.21896 at emba.uvm.edu>, brianf at med.uvm.edu (Brian
> I use glycogen in my precipitates of ds-DNA (pBlueScript) for
> sequencing and it works just fine. It has no benefit other than making
> the pellet of denatured DNA easy to see so I don't accidentally aspirate
> it out of the tube when removing the 70% EtOH. i.e. the DNA precipitates
> just fine without it, but it does not inhibit the Sequenase 2.0 enzyme.
Do you do NaOH denaturation of pDNA in presence of glycogen or you add it
later for ppt??
Is there any effect of the glycogen on the sequencing gel??
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