translucent PVDF membranes

vaughan at vaughan at
Fri Feb 18 14:50:02 EST 1994

Hello Fellow Western Blotters,
	I have been using PVDF membranes for Western blots recently, and have 
noticed that the membrane becomes translucent if "too much" protein
is loaded in the lane. 	This is a problem since the blot does not become 
opaque again after it is dried. 
	Has anyone else seen this problem, and what have you done about it?
	Thanks for any imput.
	Kevin Vaughan

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