How to avoid PCR errors?

Frederick Roth roth at husc7.harvard.edu
Fri Feb 18 20:21:32 EST 1994


It is my understanding (please correct me if I'm wrong) that Perkin Elmer 
has NO DATA on the fidelity of ULTma polymerase.  Maybe it's better than 
Taq, maybe worse.  They do have data that it is proofreading, but I would 
like to see more before counting on it's high fidelity.
		
				-Fritz Roth

>> [deleted]

>I think you're right in that Vent from NEB isn't licensed for PCR, but I do
>know that PE sells a proofreading enzyme called UlTma that is good for
>proofreading during PCR, and it is licensed. As far as I know, it would be
>good for the application described here.
>[deleted]




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