How to avoid PCR errors?
roth at husc7.harvard.edu
Fri Feb 18 20:21:32 EST 1994
It is my understanding (please correct me if I'm wrong) that Perkin Elmer
has NO DATA on the fidelity of ULTma polymerase. Maybe it's better than
Taq, maybe worse. They do have data that it is proofreading, but I would
like to see more before counting on it's high fidelity.
>I think you're right in that Vent from NEB isn't licensed for PCR, but I do
>know that PE sells a proofreading enzyme called UlTma that is good for
>proofreading during PCR, and it is licensed. As far as I know, it would be
>good for the application described here.
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