Bacterial cell death

Creg Darby cdarby at
Sat Feb 19 14:16:38 EST 1994

shiha02 at writes:

>Can anyone recommand a good bacterial system where if a gene is
>induced leads to cell death?

There's a galE mutation in E. coli that when mutated causes cells to lyse in 
the presence of galactose; but the cells grow fine without the sugar.
If I remember correctly, the cells accumulate a metabolic intermediate 
that they can't process further because of the mutation, and the 
accumulated substrate causes lysis.

Creg Darby
cdarby at
Department of Genetics SK-50, University of Washington, Seattle, WA 98117
voice: 206-543-9446   fax: 206-543-0754

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