Imagene green fluorogenic galactoside

ww40 William_D_WARREN at UMAIL.UMD.EDU
Sat Feb 19 15:07:06 EST 1994


>In article <2j6ae8$mbr at mserv1.dl.ac.uk>,
>Richard N. Day <rnd2v at galen.med.virginia.edu> wrote:
>>In response to Steve Kay's query:
>>Dear netters,
>>        I am trying to get some information on a reporter gene
>>system called Green Fluorescent Protein. If anyone has refs or
>>any other info I would greatly appreciate it.

>>Dear Steve:
>>   Are you thinking of the Green fluorogenic substrates for beta
>>galactosidase from Molecular probes?  Or is this something
>>different>

>>Rich Day
>>NSF Center for Biological Timing
>>University of Virginia
>
>
>I sent this to Steve but perhaps the rest of you might be interested.
>Martin Chalfie at Colombia developed a plasmid that contains the gene for
>jellyfish green fluorescent protein. This protein glows green in living
>cells when it is exposed to blue light. The glow is bright enough to be
>seen with the eye. The photos I saw of it in action were taken from living
>worms carrying a trangene with a tissue specific promoter linked to the
>jellyfish green protein. Single cells were filled with the protein and
>processes were easily visualized. 
>
>This reporter works in worms and is I think being used in mammal cells by
>Curan's group at Roche but I don't know about other species. As a reporter
>in transgenics or cell cultures it seems to be very promising since it is
>visualizable in living cells with minimal equipment.
>

The reference for this is Science 263: 802-805
"Green Fluorescent Protein as a Marker for Gene Expression"
M. Chalfie, Y. Tu, G. Euskirchen, W. Ward and D. Prasher.



^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^
William D. WARREN, PhD
Center for Agricultural Biotechnology     Email: ww40 at umail.umd.edu
University of Maryland at College Park    Phone: (301) 405-7681 

^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^




More information about the Methods mailing list