PCR Cloning

BIONETDEMO at BIOTECHNET.COM BIONETDEMO at BIOTECHNET.COM
Mon Feb 21 23:19:50 EST 1994


My lab is preparing to do some PCR cloning work.  Before we start, I would a
appreciate some input. 
   Does TA Cloning work well?
   Is it better to polish the ends of the PCR products and blunt-end ligate?
   Has anyone compared the senecommercial;lly-available kits?
   Are there any important tricks to be aware of?

I would appreciate any help you can give me!  Thanks in advance.



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