Pharmacia Phage Display Kit
Joe Major - Tannox
major at dino.qci.bioch.bcm.tmc.edu
Tue Feb 22 09:43:26 EST 1994
Has anyone out there used the Pharmacia Phage Display Kit? A colleague
has begun using it and following their directions implicitly has
gotten only 3,000 plaques from a total of 100-200 ng of ligated
CANTAB vector using the PCR generated insert from immunized mouse.
This is much lower than was expected. Competent cells have good
efficiency, so that is not the problem. Any hints with this or
related systems would be helpful.Thanks in advance.
Joseph G. Major, Ph.D. major at qci.bioch.bcm.tmc.edu
(713)664-2288 (voice) (713)664-8914 (fax)
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