s-s M13 cloning/sequencing

Ashleigh Mckenzie MCKENZIEA at gate2.cc.unp.ac.za
Tue Feb 22 07:38:14 EST 1994


Dear netters

Has anyone experienced loss of restriction sites in the M13mp18
or 19 polylinker after ligating a DNA sequence into either
vector? It seems too that the vectors sequence well without an
insert but recombinant M13mp18 sequences result in lanes which
fade very early and which are of different (but random)
intensities.
Can anyone suggest ways to remedy these problems?

Thanks in advance

Ashleigh Mckenzie

mckenziea at botany.unp.ac.za



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