Purification from Low Melt

T. S. Pillay tpillay at ucsd.edu
Thu Feb 24 01:19:35 EST 1994

Subject: Purification from Low Melt
From: gc, genecutl at mendel.berkeley.edu
Date: Thu, 17 Feb 1994 17:43:45 +0000
In article <genecutl-170294174345 at kos2mac16.berkeley.edu> gc,
genecutl at mendel.berkeley.edu writes:
>Lately I've been getting very inconsistent results when I try to purify
>DNA from low-melt agarose.  I've tried spinning it through glass-wool
>and freezing it and spinning it down.  Sometimes I get good yields but
>other times I find that the majority of the DNA is retained in the
>agarose.  Does anybody know what could be going on?  Are there other
>techniques which give more consistent results?

Magic PCR prep from ProMega works well in my hands for purifying DNA
(besides PCR products) from low gelling agarose.  have you tried it?

T.S. Pillay
tpillay at ucsd.edu   Tel: 619-552-8585 ext 7148  Fax: 534:-7181

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