Purification from Low Melt

T. S. Pillay tpillay at ucsd.edu
Thu Feb 24 01:19:35 EST 1994


Subject: Purification from Low Melt
From: gc, genecutl at mendel.berkeley.edu
Date: Thu, 17 Feb 1994 17:43:45 +0000
In article <genecutl-170294174345 at kos2mac16.berkeley.edu> gc,
genecutl at mendel.berkeley.edu writes:
>
>Lately I've been getting very inconsistent results when I try to purify
>DNA from low-melt agarose.  I've tried spinning it through glass-wool
>and freezing it and spinning it down.  Sometimes I get good yields but
>other times I find that the majority of the DNA is retained in the
>agarose.  Does anybody know what could be going on?  Are there other
>techniques which give more consistent results?
>Thanks.
>-- 
>--gc

Magic PCR prep from ProMega works well in my hands for purifying DNA
(besides PCR products) from low gelling agarose.  have you tried it?

T.S. Pillay
tpillay at ucsd.edu   Tel: 619-552-8585 ext 7148  Fax: 534:-7181



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