** add e-mail adresses to FAQ ?? **

Bob Ludwig lab niflab at biology.UCSC.EDU
Wed Feb 23 17:46:39 EST 1994


Actually, I`m replying to your previous post about cutting in
a ligation.  I saw a ref about 10 yrs ago that did that. I have 
tried it since whenever doing isoligamer (my word for enzymes
that ligate together but don't havve the same cutseq) ligations,
e.g., Bam-Bgl digests work fine with a little Bam and a little
Bgl thrown in.

			--b0b kuhn
(ps--miss your little logo-face--but I've seen someone else has
picked it up....)

Robert Kuhn, PhD              "Twenty years of schoolin'
Sinsheimer Laboratories        and they put you on the day shift
University of California    
Santa Cruz, CA 95064  USA      (brown shoes don't make it)"
                                           -F. Zappa (RIP) 
email: rkuhn at orchid.ucsc.edu

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