PCR AMPLIFICATION AND GAPS!!! - CORRECTION

Prasit Palitpolkanpim - GRSO grppl at MUCC.MAHIDOL.AC.TH
Thu Feb 24 18:30:43 EST 1994


Dear Dr. Prade,
	An interesting question.  I guess that the exponential 
amplication  can be done by increasing the concentration of the  
primer 1 to match the excess amount of template B.
	Regards.
	Prasit P.
On 24 Feb 1994, ROLF PRADE wrote:

> 
> 
>  	 
>         Hi Netters. This is a PCR design question.
>  	
>  	If I have the following situation, will I observe exponential, 
>  	linear or no amplification in a PCR reaction?
>  
>  	
>  	
>             	   Primer 1
>  	   	   ----->
>          	5' -----------------------------------------3' Template A
>          	   ||||||||||||||||||||||||||||||| gap ||||||
>     Template B	3' -------------------------------    <-----5'
>                                       |                 Primer 2
>                                       |
>  				     |PCR
>                                       V
>                  5'---->-------------------------------------3'
>     PRODUCT ?      ||||||||||||||||||||||||||||||||||||||||||
>  		3'------------------------------------<-----5' 
>  
>         
>   ===>	Template B is present in excess in relation to template A
> 
>  	Lets assume that everything else is optimal in the reaction.
>  
>  	Thanks in advance for the clue that I can't see.
>  
>  	Rolf
> 
> 




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