PCR AMPLIFICATION AND GAPS!!! - CORRECTION

ROLF PRADE PRADE at bscr.uga.edu
Thu Feb 24 16:41:30 EST 1994



 	 
        Hi Netters. This is a PCR design question.
 	
 	If I have the following situation, will I observe exponential, 
 	linear or no amplification in a PCR reaction?
 
 	
 	
            	   Primer 1
 	   	   ----->
         	5' -----------------------------------------3' Template A
         	   ||||||||||||||||||||||||||||||| gap ||||||
    Template B	3' -------------------------------    <-----5'
                                      |                 Primer 2
                                      |
 				     |PCR
                                      V
                 5'---->-------------------------------------3'
    PRODUCT ?      ||||||||||||||||||||||||||||||||||||||||||
 		3'------------------------------------<-----5' 
 
        
  ===>	Template B is present in excess in relation to template A

 	Lets assume that everything else is optimal in the reaction.
 
 	Thanks in advance for the clue that I can't see.
 
 	Rolf



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