PCR AMPLIFICATION AND GAPS!!!

ROLF PRADE PRADE at bscr.uga.edu
Thu Feb 24 16:22:36 EST 1994


	 
        Hi Netters. This is a PCR design question.
	
	If I have the following situation, will I observe exponential, 
	linear or no amplification in a PCR reaction?

	
	
           	   Primer 1
	   	   ----->
        	5' -----------------------------------------3' Template A
        	   ||||||||||||||||||||||||||||||| gap ||||||
   Template B	3' -------------------------------    <-----5'
                                     |                 Primer 2
                                     |
				     |PCR
                                     V
                5'---->-------------------------------------3'
   PRODUCT ?      ||||||||||||||||||||||||||||||||||||||||||
		3'------------------------------------<-----5' 

	Lets assume that everything else is optimal in the reaction.

	Thanks in advance for the clue that I can't see.

	Rolf

                                                   



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