PCR AMPLIFICATION AND GAPS!!!
ROLF PRADE
PRADE at bscr.uga.edu
Thu Feb 24 16:22:36 EST 1994
Hi Netters. This is a PCR design question.
If I have the following situation, will I observe exponential,
linear or no amplification in a PCR reaction?
Primer 1
----->
5' -----------------------------------------3' Template A
||||||||||||||||||||||||||||||| gap ||||||
Template B 3' ------------------------------- <-----5'
| Primer 2
|
|PCR
V
5'---->-------------------------------------3'
PRODUCT ? ||||||||||||||||||||||||||||||||||||||||||
3'------------------------------------<-----5'
Lets assume that everything else is optimal in the reaction.
Thanks in advance for the clue that I can't see.
Rolf
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