Stratagenes lac switch inducible expression system

Richard N. Day rnd2v at GALEN.MED.VIRGINIA.EDU
Fri Feb 25 13:18:23 EST 1994


Hello fellow netters:
   Does anyone out there have experience with the lac switch
system supplied by stratagene?  We have selected a number of
clonal cell lines expressing both the RSV vector and the
repressor protein, but have only seen a maximum of 2.5 fold
induction to 5 mM IPTG.  Have any of you tried the system and
found clonal cell lines with higher fold inductions?  Our results
could be simply not screening through enough clones.
   Thanks in advance.

Rich Day
University of Virginia
rnd2v at virginia.edu

Usual disclaimer applies.



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