Insta minipreps

dspete at helix.nih.gov dspete at helix.nih.gov
Fri Feb 25 15:09:04 EST 1994


In article <9402251345.AA14419 at mbcrr.harvard.edu>, <beverley at MBCRR.HARVARD.EDU> 
writes:
 
> Has anyone tried Insta-minipreprs from 5'-->3'?  Do they work well?
> They look to be quite fast.  I also wonder what the gel barrier they
> use for the aqueous and organic phases may be.
> 
> Thanks
> 
> Steve Beverley
> BCMP Dept.  
> Harvard Medical School

Steve,

I've used them for awhile now with good results. The gel looks like a cross 
between wax and vasoline. One caveat though, disposing of the phenol/chloroform 
is somewhat of a pain since it's neatly trapped beneath the gel. I use a wood 
applicator stick to scoop the gel to one side so that the organics can be 
poured into the waste bottle.

David Peterson
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