1002

[mrege at samba.cnb.uam.es]

Hi,

I always sterilize PEG 8000 by autoclaving a 40% solution SEPARATELY
from the solution of ammonium acetate 7M (which is autoclaved also).

I never had problems of turning yellow, and when I mix the solutions
(v/v) and use them in my phage precipitations, the results are reproductible.
(over 10E14 pfu/ml).

Hope this helps,


Josefina


Josefina Enfedaque
Dept. Microbiology 
Faculty of Pharmacy				josefina at farmacia.ub.es
Universitat de Barcelona