the lac switch inducible expression system

Richard N. Day rnd2v at GALEN.MED.VIRGINIA.EDU
Fri Feb 25 11:30:41 EST 1994


Hello fellow netters:
   Does anyone out there have any experience with the lac switch
vectors supplied by Stratagene?  We have tried making stable cell
lines with the system, but find that the clonal cell lines that
we have screen all have quite low fold inductions in response to
5 mM IPTG.  I was curious if anyone else has tried the system and
if our results are simply bad luck in selecting our clones.
Thanks for your responses in advance.
Rich Day
University of Virginia
rnd2v at virginia.edu

the usual disclaimers apply.



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