PROBLRMS WITH BIO-RAD SEQUI-GEN
mkennedy at chmeds.ac.nz
Fri Feb 25 19:27:44 EST 1994
In article <940222123429.24042 at UABCVSR.cvsr.uab.edu>, txpljfg at UABCVSR.CVSR.UAB.EDU writes:
> We have had a similar experience. Ususally some acrylamide will
> accumulate in the top reservoir when we have new people using it. The
> best solution that we have found is to remove the accumulated
> acrylamide with a bent coat hanger and pray that the platinum wire
> doesn't get busted. So far so good. Has anyone else found a better
> James F. George, Ph.D. "Back off man, I'm a scientist"
We always run 100ml or so of water into the top tank before pouring the gel;
this dilutes any overflowing acrylamide, and usually prevents it spilling, or at
least makes it sufficiently sloppy to pour out later.
I seem to recall that BIORAD recommend a dilute solution of NaOH for dissolving
polyacrylamide out of the buffer reservoir.
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-0750
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