Preferential insert orientation
BARRY J MARGULIES
bjmarg at welchlink.welch.jhu.edu
Sat Jan 1 22:02:58 EST 1994
In article <gregoryh-271293155836 at 22.214.171.124> gregoryh at bcm.tmc.edu (Gregory R. Harriman) writes:
> Nonetheless, I only get colonies with the original
How many colonies did you pick to screen? Even with the worst of luck
(which it seems you may be having; did you do the ritual, obligatory
cloning dance to the Molecular Biology Gods before you started :-)), if I
pick twelve colonies (i.e., one microfuge rotor's worth), I can get my
orientation in at least 1 colony. Actually, to date, my worst luck has
been 3/12 correct orientation.
To answer the other part of your original question: Never heard of
"directional preference" with identical sites.
-Barry (bjmarg at welchlink.welch.jhu.edu)
More information about the Methods