Using DNA markers to check size of RNA
BARRY J MARGULIES
bjmarg at welchlink.welch.jhu.edu
Sat Jan 1 21:57:49 EST 1994
In article <CIpGxt.BGH at umdnj.edu> bawagan at umdnj.edu (Hinayana Bawagan) writes:
> I find it a pain to run RNA formaldehyde gels. Even with 3 uls of RNA, I
>get the faintest signal.
There's an article in Biotechniques that describes the best way to see
RNA with ethidium. I think it's from within the last 2 years or so
(maybe Jan. 91) and one of the authors is Gong.
> I can use 0.5% TBE agarose gel but my problem is with DNA markers, I can
>only say that my RNA migrates with this band of DNA. Can I do something to
>the DNA markers that will make them run like a ss RNA? Has anyone out there
>establish that a single-stranded RNA of a particular size migrates with
>DNA of a particular size?
Many companies sell RNA markers. I have used the ones from BRL, which
work well. Hope this helps.
-Barry (bjmarg at welchlink.welch.jhu.edu)
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