Solubilising PAGE gels (probably a FAQ)

Ron Kagan rkagan at ewald.mbi.ucla.edu
Tue Jan 4 20:29:06 EST 1994


In article <2gbvibINNj44 at s-crim1.dl.ac.uk> R.G. Walters,
mbrgw at s-crim1.dl.ac.uk writes:

>I want to cut-out-and-count bands from a PAGE gel, and would like a
quick-and-
>easy method for solubilising the acrylamide.  I seem to recall a method 
>involving H2O2, but don't know the details.  Can anyone help?

H2O2 works, but you can also use _SOLVABLE_(TM) from NEN Research Products
 (Cat. # NEF-910).  It is a tissue and gel soulbilizer and it works great
for dissolving acrylamide gel slices for scintillation counting.



>Cheers,
>
>Robin Walters.                      Robert Hill Institute, Sheffield UK.
>
>A fact is an opinion that everyone agrees with.

No, reality is objective; facts exist independently of anyone's beliefs,
fears, or wishes.

    "If one man is more right than his fellow men, then he is a majority
of one."

                                                                         
           - Henry David Thoreau.


Regards,

Ron Kagan
rkagan at ewald.mbi.ucla.edu



More information about the Methods mailing list