Trouble growing EMBL4 to high titer

at reks.uia.ac.be at reks.uia.ac.be
Wed Jan 5 12:07:06 EST 1994


In article <94005.100228RROXBY at MAINE.MAINE.EDU> Robert Roxby <RROXBY at MAINE.MAINE.EDU> writes:
>I would appreciate any hints about how to grow EMBL4 to high
>titer.  This is the intact EMBL4, not a recombinant.  I'm
>using LE392 as the host and a rich medium, NZC.  I'm getting
>titers of about 2e9/mL, but I am led to expect about 10 times
>that amount, as a minimum, by the various manuals.
>
>Any advice greatly appreciated.
>Bob

Hi!
I have similar problem with clones in EMBL4. Any hints
would be greatly appreciated.
What I am doing at the moment is the plate lysis (see Maniatis).
I works OK but still I need a few large plates to get enough
to work with.
So, any hints about the liquid cultures would be welcome.
Przemko




More information about the Methods mailing list