Trouble growing EMBL4 to high titer

Robert Roxby RROXBY at MAINE.MAINE.EDU
Wed Jan 5 10:02:28 EST 1994


I would appreciate any hints about how to grow EMBL4 to high
titer.  This is the intact EMBL4, not a recombinant.  I'm
using LE392 as the host and a rich medium, NZC.  I'm getting
titers of about 2e9/mL, but I am led to expect about 10 times
that amount, as a minimum, by the various manuals.

Any advice greatly appreciated.
Bob



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