Primer Extension Problem!
leach at mbcrr.harvard.edu
Thu Jan 6 06:28:23 EST 1994
In article <1994Jan6.092453.1 at omrf.uokhsc.edu>, frank at omrf.uokhsc.edu
> We have tried to map the transcription start site of a gene using the
> primer extension method without success. We obtain a number of bands, but
> all of these bands appear on the autoradiograph whether we load a sample
> of just the primer or the reverse transcribed RNA starting at the primer.
> We are using 3 primers at different positions and the problem occurs with
> all three primers. We would greatly appreciate hearing from anyone who
> has had this problem and how they resolved it.
> Many thanks,
> Bart Frank
Have you tried a blank piece of film??
Maybe the intensifying screens are contaminated with radiation.
..... Martin Leach Email:leach at mbcrr.harvard.edu
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