Primer Extension Problem!

Martin Leach leach at
Thu Jan 6 06:28:23 EST 1994

In article <1994Jan6.092453.1 at>, frank at

> We have tried to map the transcription start site of a gene using the
> primer extension method without success. We obtain a number of bands, but
> all of these bands appear on the autoradiograph whether we load a sample
> of just the primer or the reverse transcribed RNA starting at the primer.
> We are using 3 primers at different positions and the problem occurs with
> all three primers.  We would greatly appreciate hearing from anyone who
> has had this problem and how they resolved it.
> Many thanks,
> Bart Frank

Have you tried a blank piece of film??

Maybe the intensifying screens are contaminated with radiation.


.....          Martin Leach                Email:leach at 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
               USA                           head.....WIBBLE" -BLACKADDER  

More information about the Methods mailing list