promoter analysis

[Martin Leach]

In article <CJ7FL1.I85 at biobase.aau.dk>, sjn at biobase.aau.dk (Soeren Jensby
Nielsen) wrote:

> Hi Netters!
> 
> I am about to make a deletion analysis of my human promoter, and
> since we have no local expertise in this field I would be happy
> for any suggestions/answers to the following questions.
> 
> 1)   What is your preferred reporter gene - and assay?

firefly luciferase
> 
> 2)   Have you got a favorite vector, and where can one get hold
>      of it?

genelight vectors/promega
> 
> 3)   Do you use an internal transfection standard, or normalize
>      to total protein content.

i do protein assays, works well
> 
> I might add that this poor university is not equipped to do luci-
> ferase assays, so light is out of the question.

can use a scintillation counter with a fitted single photon monitor
> 
> Thanks for any help offered.
> 
> Soeren.

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
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