leach at mbcrr.harvard.edu
Thu Jan 6 06:25:43 EST 1994
In article <CJ7FL1.I85 at biobase.aau.dk>, sjn at biobase.aau.dk (Soeren Jensby
> Hi Netters!
> I am about to make a deletion analysis of my human promoter, and
> since we have no local expertise in this field I would be happy
> for any suggestions/answers to the following questions.
> 1) What is your preferred reporter gene - and assay?
> 2) Have you got a favorite vector, and where can one get hold
> of it?
> 3) Do you use an internal transfection standard, or normalize
> to total protein content.
i do protein assays, works well
> I might add that this poor university is not equipped to do luci-
> ferase assays, so light is out of the question.
can use a scintillation counter with a fitted single photon monitor
> Thanks for any help offered.
..... Martin Leach Email:leach at mbcrr.harvard.edu
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