Soeren Jensby Nielsen
sjn at biobase.aau.dk
Thu Jan 6 05:53:24 EST 1994
I am about to make a deletion analysis of my human promoter, and
since we have no local expertise in this field I would be happy
for any suggestions/answers to the following questions.
1) What is your preferred reporter gene - and assay?
2) Have you got a favorite vector, and where can one get hold
3) Do you use an internal transfection standard, or normalize
to total protein content.
I might add that this poor university is not equipped to do luci-
ferase assays, so light is out of the question.
Thanks for any help offered.
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