unmethylated lambdaZAP DNA ?

Steven Russell Genetics sr120 at mbfs.bio.cam.ac.uk
Fri Jan 7 03:47:33 EST 1994


In article <2ghbb7$adf at hermod.uio.no> CASPERL at BIOMED.UIO.NO writes:

>I am working on a barley gene promoter and want to make several constructs
>(in pBluescript) containing different promoter fragments hooked up to Gus-Nos.
>
>My barley gene is cloned in lambda ZAP and the DNA prep is methylated as we
> used
>BB4 cells.
>
>In the subcloning it would be very handy to use BclI (dam sensitive) and to my
>knowledge there are no dam insensitive isoschizomers of BclI.

Why don't you just zap out bluescript from the phage vector and then pass the 
plasmid through a dam- strain?

Steve Russell
Dept of Genetics
University of Cambridge
UK
SR120 at mbfs.bio.cam.ac.uk



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