plasmid integration after transfection

Martin Kennedy mkennedy at
Thu Jan 6 18:41:38 EST 1994

In article <2gglat$h34 at>, suter at writes:
> question: if one transfects a mammalian cell with a plasmid, how many copies 
> will integrate in the genome ? is this dependend on the procedure followed ?
> cheers,
> clemens


The number of plasmids that integrate depends very strongly on the method used
to intrduce the DNA into the cell, for reasons I don't fully understand.  I
know that with electroporation (into mouse ES cells, at least) I generally get
a single copy insertion, and about 10% of the cells have two discrete
insertions.  Very rarely we see more than 2.  With CaPO4 and various other
chemical means, it seems that many more copies integrate, often as concatamers.



NNNN   NN  Martin A Kennedy (E-mail = mkennedy at  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
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