CsCl-EtBr purification

Frances Hannan Zoology flh at mbfs.bio.cam.ac.uk
Fri Jan 7 08:37:43 EST 1994


muriana at aclcb.purdue.edu (Peter M. Muriana) writes:

>In an old post (ca. mid December) I mentioned the use of isopropanol vs. 
>butanol in extracting EtBr.  

>An alert Michael Tuvin pointed out that I mentioned the use of n-butanol as
>not being completely miscible with water, and that it should be 
>iso-/sec-butanol.  I stand corrected; thank-you Michael.

>Regards, Peter Muriana

I have always used n-butanol for extracting EtBr from plasmid preps.
Maniatis also says 1-butanol NOT 2-butanol....

Frances Hannan
AFRC LMS, Zoology, Cambridge
flh at mbfs.bio.cam.ac.uk



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