Purifying Chicken IgY

David M. Berman images at netcom.com
Mon Jan 10 07:33:47 EST 1994


Has anybody tried the chloroform/PEG method of antibody purification
from egg yolk?  Do these antibodies perform well?  The method (Polson,
1990, J Imm Inv) is easy and gives good yields but the original
article uses an Ouchterlony assay as the only proof of Ig function --
I'm a little concerned about possible effects of chloroform on the Abs
performance in sandwich assays.
I'd appreciate any feedback from anyone with experience in these
matters.

David Berman
UT Southwestern Medical School
Dallas, TX 
USA



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