Mg and PCR
annag at vifp.monash.edu.au
Tue Jan 11 23:04:23 EST 1994
Can anyone explain or give me a reference on why magnesium produces extra bands
when it is in excess in the PCR reaction? One suggestion has been that primers bind non-specifically to other DNA regions and so produce extra bands in the PCR reaction.
Why should this happen whem Mg is in excess?
Thanks in advance.
Victorian Institute of Forensic Pathology,
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