Mg and PCR

Anna Georgalis annag at vifp.monash.edu.au
Tue Jan 11 23:04:23 EST 1994


Can anyone explain or give me a reference on why magnesium produces extra bands 
when it is in excess in the PCR reaction? One suggestion has been that primers bind non-specifically to other DNA regions and so produce extra bands in the PCR reaction.
Why should this happen whem Mg is in excess?                                                   

Thanks in advance.

Anna Georgalis
Victorian Institute of Forensic Pathology,
Melbourne,Australia.





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