Episome Transfer

Seth Findley findley at u.washington.edu
Tue Jan 11 19:10:13 EST 1994

Dose anybody have a recommended protocol for transfer of a 60kb episome 
from one strain to another (F-) strain?  The episome is selectable 
(kanamycin rersistance)

Could I just do an alkaline miniprep on the episome-containing strain and 
electroporate the F- strain?

Any tips appreciated!


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