Home made gems don't "invert"
denomme at fhs.csu.McMaster.CA
Wed Jan 12 13:20:11 EST 1994
After one purchase of PCR GEMS, I thought I would make my own wax gems. I
did a wais search and found that some people use parowax from the local
grocery store. I made some pellets (about the same weight as the
commercial ones) and tried them out. I want a true 'hot-start' (ie. lower
mix - wax plug - upper mix) but upon heating the wax doesn't invert with
the upper mixture, ie. t the end of the thermalcycling the wax is not on the
top. I tried parawax from a histology lab with the same result.
Could someone comment on how to get home made gems to work. If you use
a particular wax please be specific.
Thanks in advance.
denomme at fhs.csu.mcmaster.ca
Department of Pathology
Hamilton, Ontario, CANADA
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