Contaminating plasmid

Abiye Iyo aiyo at uoguelph.ca
Wed Jan 12 12:49:38 EST 1994


Hi,
	It is possible that the original stock you had was already
contaminated.  My humble suggestion is that you chuck out all your plasmid
stocks that seem to be contaminated and re-order new vectors from the
manufacturers.  Also make sure your solutions are not contaminated by
plamids from previous use ie start with sterile solutions and avoid
contamination.
	Another thing to do will be to transform E. coli with dilute
plasmid prep and screen transformants individually.  Seems kind of a long
way to go.
	Anyway good luck!

Abiye





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