Contaminating plasmid

Christian Geltinger geltinger at gsf.de
Wed Jan 12 13:07:39 EST 1994


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Subject: Contaminating plasmid
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A number of us have had problems with a plasmid which seems to
contaminate our plasmid stocks. It's ampicillin resistant and
has a linear size of around 2.2 kb. The strangest aspect is the
lack of sites for common restriction enzymes. It has an SstI (SacI)
site but lacks sites for BamHI, HindIII, KpnI, PstI, EcoRI, SalI
and EcoRV.

It often appears as a faint band in preps of plasmid DNA from
constructs in pUC and Bluescript type vectors.

Can anybody suggest what it might be? I'd be very grateful for
suggestions.

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  Dr Raymond Dalgleish,          JANET: ray at uk.ac.le
  Department of Genetics,     INTERNET: ray at le.ac.uk
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