Help needed to clone a gene

mingfeng at kean.ucs.mun.ca mingfeng at kean.ucs.mun.ca
Wed Jan 12 09:05:12 EST 1994


Hi, there, I got a protein that can specifically bind to a known DNA
sequence. Now I want to clone the gene of this protein use this DNA
fragment. Do you guys have any suggesttion that I should go directly 
to screen the cDNA expressing library or to purify this protein with
DNA fragment first and then clone the gene? I am just wondering which
way is better and easier. 
Any help is appreciated. Thanks in advance.
e-mail: mingfeng at kean.ucs.mun.ca




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