help problem with lambda

the End jgraham at bronze.ucs.indiana.edu
Wed Jan 12 15:29:01 EST 1994


Reliable medium scale Lambda preps are best in my experience when based on
liquid lysis rather than plate techniques. Try the procedure suggested by 
Dexter Chisholm at Dupont as reported in "Biotechniques" Vo.7 no.1 1989 p. 21.
This anaylsis of the relevant parameters is the definitive word on producing
workable Lambda DNA IMHO.

Best wishes,

Jim
J. Graham
Biology and Chemistry Departments
Indiana University Bloomington





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