transformation with denatured plasmids

unknown at dl.ac.uk unknown at dl.ac.uk
Fri Jan 14 12:22:56 EST 1994


Hi there,
I have a stupid question about plasmid transformation. If plasmid DNA
is denatured, say by heating, dose it still have a ability to
transform host bacteria? What will happen if renatured plasmid DNA is
used? If renatured DNA can transform host, what is the efficiency?

Thanks in advance.

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Peijun Zhang                      #                          
Department of Plant Science       #           
University of Manitoba            #     
Winnipeg, MB                      #    
R3T 2N2                           #       
Tel:                              #               
Fax:                              #
E-mail: pzhang at cc.umanitoba.ca    #
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