Protein/DNA isolation

Mon Jan 17 06:27:34 EST 1994

> >I was wondering if there were protocols out there which allowed
> >you to extract both the DNA and the protein from the same tissue.

> In the last Bio/technology there is an advertisement about TRI 

There is another's that wonder reagent C6H6O (phenol to you 
non-chemistry majors)!  You simply do your phenol extraction of liq 
N2-frozen and ground tissue (we use cheap coffee-grinders) - then 
SAVE THE PHENOL PHASE, because that is where your proteins (or the 
large bulk of them) will be...!  You can wash this free of insoluble 
guck, then extract with 5 vol of ice-cold methanol (protein pptes).

 | Ed Rybicki, PhD             |                                      |
 | (ed at        |      "Lord, won't you buy me         |
 | Dept Microbiology           |          A Mer-ce-des Benz           |
 | University of Cape Town     |     My friends all have Porsches     |
 |                             |          I must make amends..."      |                      |
 | Private Bag, Rondebosch     |                                      |
 | 7700, South Africa          |           - Janis Joplin             |
 | fax: xx27-21-650 4023       |            (Pearl, 1971)             |
           First Albert, now Frank...go well, axemen!

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