About cycle sequencing

BHWFDUNG at cphkvx.cphk.hk BHWFDUNG at cphkvx.cphk.hk
Mon Jan 17 06:17:33 EST 1994


Dear Netters,

I have used the Promega fmol seqencing kit for doing plasmid sequencing, but I
always get multiple-band in four lanes.  When I used the traditional method
like the T7 DNA Polymerase Sequencing kit (Pharmarcia), with the same DNA
sample and primer, there is no such problem.  I just want to know whether
anybody has got the same problem and known what is the reason for that.

Thank you for your help!

e-mail address:
bhwfdung at cphkvx.cphk.hk

Dept of Biology and Chemistry
CPHK
17/1/94




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