dsRNA transfer
Morag Lancaster
morag at arbo.microbiol.uwa.edu.au
Wed Jan 19 09:09:07 EST 1994
Hi,
I am having problems with the transfer of ds RNA from agarose gels to
nitrocellulose membrane. I have tried several methods previously posted on
the net however although the smaller frags transfer readily the higher
molecular weight markers and my ds RNA ( ~11kb) does not seem to transfer ( I
restain my gel after transfer to determine the success of transfer). Does
anyone have any suggestions? I am denaturing in 50mM NaOH for 30min. Thanks.
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