YEAST TWO-HYBRID//INTERACTION TRAP

[Seth Findley]

A neophyte to the yeast-based Two-Hybrid (Interaction Trap) system would 
like to know:

What is the limit of detection for this system?
That is, what is the weakest bona fide interaction detectable above 
background?
(I know I am asking for trouble with this question)

For Instance: have many potential (growth factor) ligands been used to find 
their receptors (or vice versa)?

Thanks,

SDF

U of W

Seattle