Air Thermo Cycler ????
James N Petitte
jnppo at unity.ncsu.edu
Tue Jan 18 14:01:15 EST 1994
carlos h. pedemonte (pedemonte at Jetson.UH.EDU) wrote:
: Has anybody tried the 1605 Air Thermo Cycler from Idaho
: Technology. I would like to know about the pros and cons of the instrument
: as well as the handling of capillary tubes, contamination problems etc.
: I would be grateful for any comment. Carlos
: Carlos H. Pedemonte Telephone: 713-743-1211 (office)
: Dept. of Pharmacology 713-743-1228 (lab)
: University of Houston Fax: 713-743-1229
: Houston, TX 77204-5515 e-mail: pedemonte at jetson.uh.edu
Yes, we have had the air thermocycler for about 1.5-2 years now. It is
definately fast, no question about it. For our purposes we needed a way
to determine the sex of unincubated chick embryos that would allow time
to manipulate the embryo in the same morning. We amplify a W
chromosome repetitive element that is repeated about 10,000 times. The
PCR is finished in 15-20 minutes and the whole procedure takes about 2.5
hours, i.e. from embryo biopsy through electrophoresis. The reactions
are in 10ul so it also saves on reagents. The buffer contains ficoll and
the reaction is loaded directly from the capplillary tube to the gel. The
company also supplies a series of buffers for optimization which has
come in handy.
On the down side, adapting traditional PCR conditions to the
cappliary-tube system is not straight forward. Some lag time is
If you really need the speed, it cannot be matched.
We also have a conventional PCR thermocycler. My recommendation is that
it can supplement but not replace a microtube-based system.
J_Petitte at NCSU.EDU
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