protein expression

[unknown at dl.ac.uk]

You should look into expression in mammalian cells using Sindbis viral
vectors. It is possible to get very high levels of expression and of course,
proper glycosylation. Some references:
Xiong, C. et al. Science V243 1188-1191
Bredenbeek, PJ and Rice, CM. Sem. Vir. V3 297-310
Hahn, CS, et al. PNAS V89 2679-2683
Bredenbeek, et al. J. Vir. V67 6439-6446

Brett Lindenbach
brett at bocim.wustl.edu