Ligation - dephosphorylation?

KLADE Torsten torsten at
Thu Jan 20 04:16:40 EST 1994

To all netters!

Is it absolutely necessary to dephosphorylate the target vector
when ligating single digested inserts?
I kept dephosphorylation with additional Phenol-Chloroform extraction, 
but the efficience of the ligation didn`t seem to improve!
Any sugestions?

Thank you

Torsten Klade torsten at
University of Salzburg

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